We will exploit the current knowledge of structural and mechanistic properties of monooxygenases and oxidases to develop enzyme redesign to deliver robust enzymes using state-of-the art methodologies for predicting beneficial mutations, creating mutant libraries and developing effective screening methods. As well as tuning existing monooxygenases and oxidases towards robust biocatalysts some novel oxidative biocatalysts (alcohol oxidases and dehydrogenases) will be identified using sequence and structural information.
The research in Work Package 1 will result in the generation of a set of robust, productive and selective oxidative biocatalysts for which target reactions of interest for industrial applications have been identified for development in ROBOX. The reactions involve the generation and/or oxidation of alcohols through selective oxidations. The ROBOX biocatalysts generated in this Work Package will be delivered to the partners in the subsequent Work Packages where they will be evaluated and scaled-up for use in oxidation reactions.
DSM Chemical Technology R&D BV, Dyadic Nederland BV, c-LEcta GmbH, Evocatal GmbH, Technical University Graz, University of Groningen, University of Pavia, Rheinisch-Westfälische Technische Hochschule Aachen, University of Manchester, Fraunhofer Gesellschaft zur Förderung der Angewandten Forschung EV, Eidgenössische Technische Hochschule Zurich, InnoSyn R&D.
Lead contact: Prof.Dr.Ir. Marco W. Fraaije, University of Groningen