ROBOX: Expanding the industrial use of Robust Oxidative Biocatalysts for the conversion and production of alcohols

The ROBOX Project

Oxygen functionalities are key functional groups in many of today’s chemicals and materials. The efficient introductions of oxygen-functionalities into raw materials are key chemical transformations in bulk and fine chemicals. Innovative bio-catalytic oxidation routes using molecular oxygen (from air) under benign and mild (pH) conditions such as ambient temperature and pressure can greatly improve the sustainability and economics of processes, but are so far mainly been applied in the pharma segments. In this segment, the enzyme-catalyzed step often represents the highest added value and the high price of the end-product (> €1000/kg) justifies less than optimal enzyme production and limitations in its catalytic efficiency. In order to achieve the widening of industrial application of enzymatic bio-oxidation processes to also larger volume but lower price chemical markets, ROBOX will demonstrate the techno-economic viability of bio-transformations of four types of robust oxidative enzymes: P450 monooxygenases (P450s), Baeyer-Villiger MonoOxygenase (BVMOs), Alcohol DeHydrogenase (ADH) and Alcohol OXidase (AOX) for which target reactions have already been validated on lab-scale in pharma, nutrition, fine & specialty chemicals and materials applications.

The introduction of ROBOX bio-oxidation processes is expected to bring substantial reductions in cost (up to -50%), energy use (-60%), chemicals (-16%) and GHG-emissions (-50%).

Work Packages

The ROBOX project has an integrated work-flow structure of 7 work packages.


The intensive networking with our scientific partners enables us to keep at the front line of top-level research and strengthens the knowledge base in the field of industrial biotechnology.

ROBOX Publications

Read articles from our news archive, newsletters and brochures, ROBOX publications, and deliverable reports.

Our Partners

Latest news

ROBOX Fused enzyme paper gets VIP paper on @ChemistryViews

“Design of Artificial Alcohol Oxidases: Alcohol Dehydrogenase–NADPH Oxidase Fusions for Continuous Oxidations” paper by our ROBOX partners at RUG report the creation of the bifunctional fusion enzymes and these were found to be efficient biocatalysts to perform alcohol oxidations, merely requiring a catalytic amount of cofactor and molecular oxygen. By fusing these redox enzymes, the…

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Development and scale-up of efficient biocatalytic oxidations using oxygen

See Martin Schürmann from our partner InnoSyn present- at our “Development and scale-up of efficient biocatalytic oxidations using oxygen”H2020 EU New developments in Industrial Biocatalysis demonstration workshop” He will present results of scaled up use of molecular oxygen (from air) the cheapest and most atom efficient oxidant available for the introduction and further oxidation of…

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