As part of our activities at CPhI our partner TUG promoted the stable lyophilised CYP450 products developed as part of ROBOX. Now on offer to industry is robust CYPs for screening and upscaling from the Austrian Centre of Industrial Biotechnology (ACIB) from within the ROBOX are lyophilised CYP biocatalysts that retain full activity – even…
The ROBOX Project
Oxygen functionalities are key functional groups in many of today’s chemicals and materials. The efficient introductions of oxygen-functionalities into raw materials are key chemical transformations in bulk and fine chemicals. Innovative bio-catalytic oxidation routes using molecular oxygen (from air) under benign and mild (pH) conditions such as ambient temperature and pressure can greatly improve the sustainability and economics of processes, but are so far mainly been applied in the pharma segments. In this segment, the enzyme-catalyzed step often represents the highest added value and the high price of the end-product (> €1000/kg) justifies less than optimal enzyme production and limitations in its catalytic efficiency. In order to achieve the widening of industrial application of enzymatic bio-oxidation processes to also larger volume but lower price chemical markets, ROBOX will demonstrate the techno-economic viability of bio-transformations of four types of robust oxidative enzymes: P450 monooxygenases (P450s), Baeyer-Villiger MonoOxygenase (BVMOs), Alcohol DeHydrogenase (ADH) and Alcohol OXidase (AOX) for which target reactions have already been validated on lab-scale in pharma, nutrition, fine & specialty chemicals and materials applications.
The introduction of ROBOX bio-oxidation processes is expected to bring substantial reductions in cost (up to -50%), energy use (-60%), chemicals (-16%) and GHG-emissions (-50%).
The ROBOX project has an integrated work-flow structure of 7 work packages.
The intensive networking with our scientific partners enables us to keep at the front line of top-level research and strengthens the knowledge base in the field of industrial biotechnology.
On Saturday 6th of October ROBOX presented at the Day of Chemistry at Chemelot in Geleen (NL) and Maastricht University presented to an open general audience. In total there were 2500 visitors and the ROBOX attracted interest in the area of biotechnology. poster Dr Katrien Bernaerts presents ROBOX to a wider audience. Chemelot stands for…
Recently ROBOX oxidation technology was recently widely publicized at the leading pharmaceutical industrial showcase exhibition CPhI in Madrid. Led by our partner TUG this was an excellent opportunity to promote our use of CYP450 technology to produce pharmaceutically relevant metabolites. CLE also promoted their combination of ADH and NOX technology for alcohol oxidation. IF YOU…