ROBOX: Expanding the industrial use of Robust Oxidative Biocatalysts for the conversion and production of alcohols

The ROBOX Project

Oxygen functionalities are key functional groups in many of today’s chemicals and materials. The efficient introductions of oxygen-functionalities into raw materials are key chemical transformations in bulk and fine chemicals. Innovative bio-catalytic oxidation routes using molecular oxygen (from air) under benign and mild (pH) conditions such as ambient temperature and pressure can greatly improve the sustainability and economics of processes, but are so far mainly been applied in the pharma segments. In this segment, the enzyme-catalyzed step often represents the highest added value and the high price of the end-product (> €1000/kg) justifies less than optimal enzyme production and limitations in its catalytic efficiency. In order to achieve the widening of industrial application of enzymatic bio-oxidation processes to also larger volume but lower price chemical markets, ROBOX will demonstrate the techno-economic viability of bio-transformations of four types of robust oxidative enzymes: P450 monooxygenases (P450s), Baeyer-Villiger MonoOxygenase (BVMOs), Alcohol DeHydrogenase (ADH) and Alcohol OXidase (AOX) for which target reactions have already been validated on lab-scale in pharma, nutrition, fine & specialty chemicals and materials applications.

The introduction of ROBOX bio-oxidation processes is expected to bring substantial reductions in cost (up to -50%), energy use (-60%), chemicals (-16%) and GHG-emissions (-50%).

Work Packages

The ROBOX project has an integrated work-flow structure of 7 work packages.


The intensive networking with our scientific partners enables us to keep at the front line of top-level research and strengthens the knowledge base in the field of industrial biotechnology.

ROBOX Publications

Read articles from our news archive, newsletters and brochures, ROBOX publications, and deliverable reports.

Our Partners

Latest news

An Enzyme Cascade Synthesis of Vanillin

ROBOX has been developing scaleable routes to improved routes to vanillin (see our publications) This report by University of Stuttgart demonstrates the great interest in using oxidative enzymes to produce vanillin. Vanillin is one of the most widely used flavouring compounds worldwide as a flavour ingredient for food and beverages as well as a fragrance…

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ROBOX helps create Biotech jobs.

Our ROBOX partner Givaudan who are committed to growing their efforts in the areas of biotechnology and biocatalysis especially after the success of the ROBOX project are currently searching for a (Senior) Research Scientist (PhD), specialized in fermentation, and a Senior Research Technician (also suitable for BSc or MSc), specialized in fermentation and/or biocatalysis: To…

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Detailed Factsheets for ROBOX Reactions and Enzymes

To find out more about ROBOX enzymes and reactions read these detailed one page descriptions of enzyme characterizations or reaction parameters. ROBOX Technology Information 1. P450 Aromatic Hydroxylations 2. P450 API Metabolites on Larger Scale 3. P450 Hydroxylation of Alkenes 4. BVMO oxidation of trimethyl-cyclohexanone 5. BVMO oxidation of cyclopentadecanone 6. AOX vanillin synthesis from…

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