Our WP3 leader Gregorio Álvaro Campos explains the background and approach of “Process design and validation” work package in our latest video.
In our latest video Prof Anton Glieder explains the objectives and methods that we used to overcome the challenges to produce the ROBOX oxidation enzymes.
ROBOX has this information video with coordinator Marco Fraaje introducing the project and delivering an overview of WP1 Enzyme engineering and identification of oxidative enzymes Aim We will exploit the current knowledge of structural and mechanistic properties of monooxygenases and oxidases to develop enzyme redesign to deliver robust enzymes using state-of-the art methodologies for predicting…Details
See the online version of our paper from RUG
What to sacrifice? Fusions of cofactor regenerating enzymes with Baeyer-Villiger monooxygenases and alcohol dehydrogenases for self-sufficient redox biocatalysis
Our partners from Graz have published this useful video explaining their work on Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies For the first time this method describes reliable experimental procedures for efficient inducible methanol-free recombinant protein production in Pichia pastoris (Komagataella phaffii), employing the carbon source regulated promoters PDC and PDF in small…Details
ROBOX latest results describe the use of supported TmCHMO that was successfully immobilized and co-immobilized with GDH-Tac for the first time onto an amino functionalized support. this was demonstrated in a new process for the enzymatic oxidation of 3,3,5-trimethylcyclohexanone by co-immobilized TmCHMO and GDH-Tac. The co-immobilized biocatalyst was re-used up to 15 cycles reaching 83%…Details
Our ROBOX partners ACIB and TUG have published a paper describing the interesting hydroxylation of the common drug Ibuprofen.
Cytochrome P450 mediated hydroxylation of ibuprofen using Pichia pastoris as biocatalyst
If you are interested in learning more about this useful technology contact our supplier partner Bisy
ROBOX biocatalyis is demonstrated to be greener than chemical methods by our partner UM that for synthesis of a ROBOX target reaction that reduces the climate change impact by 71%!. This chemistry has applications in everyday uses such as printer inks and other applications. “A prospective life‐cycle assessment (LCA) of monomer synthesis: Comparison of biocatalytic…Details
“Design of Artificial Alcohol Oxidases: Alcohol Dehydrogenase–NADPH Oxidase Fusions for Continuous Oxidations” paper by our ROBOX partners at RUG report the creation of the bifunctional fusion enzymes and these were found to be efficient biocatalysts to perform alcohol oxidations, merely requiring a catalytic amount of cofactor and molecular oxygen. By fusing these redox enzymes, the…Details
See Martin Schürmann from our partner InnoSyn present- at our “Development and scale-up of efficient biocatalytic oxidations using oxygen”H2020 EU New developments in Industrial Biocatalysis demonstration workshop” He will present results of scaled up use of molecular oxygen (from air) the cheapest and most atom efficient oxidant available for the introduction and further oxidation of…Details