A hydroquinone-specific screening system for directed P450 evolution

Read our publication on this topic. It describes the direct hydroxylation of benzene to hydroquinone (HQ) under mild reaction conditions is a challenging task for chemical catalysts. A screening protocol designed for screening of P450 variants capable of producing hydroquinones. The hydroquinone quantification assay is based on the interaction of 4-nitrophenylacetonitrile (NpCN) with hydroquinones under…