Experimental Protocols for Generating Focused Mutant Libraries and Screening for Thermostable Proteins

Our partner RUG have published a detailed protocol to generate mutant libraries experimentally by primer design, mutagenesis, protein production and screening and then mutation combination strategies that can yield stabilized mutants with increases in apparent melting temperature of 20–35°C and vastly increased half-lives with resistance to cosolvents. These mutants were designed using algorithms to predict…