Here you find the latest news about this project ‘H2020robox’.
In this latest video our Work Package 5 (Process benchmarking & evaluation) leader John Woodley describes our analysis of the successes. (WP5 – Benchmarking and evaluation of new process for the production of chemicals is an essential stage in the development of a new chemical product and/or production route. For established chemical technology using conventional…
Our partner TU Graz hosted a school visit – Basics of Biotechnology for 13-14 year old school scientists. In three exercises, they experienced different laboratory techniques: streaking and growing microorganisms like bakers yeast, isolating DNA from fruits and vegetables and simulating a protein engineering exercise by doing a colorimetric assay on active and inactive clones.…
Organised by ROBOX last week ROBOX and CarbaZymes delivered their joint workshop on these two EU H2020 projects both aimed at making enzymes more available to industry. All the major enzymes delivered in both these projects were discussed and presented to an audience of 75 scientists with a large presence on industrial representatves kenly interested…
Our WP3 leader Gregorio Álvaro Campos explains the background and approach of “Process design and validation” work package in our latest video.
In our latest video Prof Anton Glieder explains the objectives and methods that we used to overcome the challenges to produce the ROBOX oxidation enzymes.
ROBOX has this information video with coordinator Marco Fraaje introducing the project and delivering an overview of WP1 Enzyme engineering and identification of oxidative enzymes Aim We will exploit the current knowledge of structural and mechanistic properties of monooxygenases and oxidases to develop enzyme redesign to deliver robust enzymes using state-of-the art methodologies for predicting…
See the online version of our paper from RUG
What to sacrifice? Fusions of cofactor regenerating enzymes with Baeyer-Villiger monooxygenases and alcohol dehydrogenases for self-sufficient redox biocatalysis
Our partners from Graz have published this useful video explaining their work on Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies For the first time this method describes reliable experimental procedures for efficient inducible methanol-free recombinant protein production in Pichia pastoris (Komagataella phaffii), employing the carbon source regulated promoters PDC and PDF in small…
ROBOX latest results describe the use of supported TmCHMO that was successfully immobilized and co-immobilized with GDH-Tac for the first time onto an amino functionalized support. this was demonstrated in a new process for the enzymatic oxidation of 3,3,5-trimethylcyclohexanone by co-immobilized TmCHMO and GDH-Tac. The co-immobilized biocatalyst was re-used up to 15 cycles reaching 83%…
Our ROBOX partners ACIB and TUG have published a paper describing the interesting hydroxylation of the common drug Ibuprofen.
Cytochrome P450 mediated hydroxylation of ibuprofen using Pichia pastoris as biocatalyst
If you are interested in learning more about this useful technology contact our supplier partner Bisy