Our partners from Graz have published this useful video explaining their work on

Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies

For the first time this method describes reliable experimental procedures for efficient inducible methanol-free recombinant protein production in Pichia pastoris (Komagataella phaffii), employing the carbon source regulated promoters PDC and PDF in small scale experiments while cultivation parameters can be monitored online, and a constant glycerol feed is applied.