During the course of ROBOX a number of public deliverables have become available which describe more details. The ones below give an ovderview of the technical work in the ROBOX project-
Completion of benchmarking protocol and flowsheets established for the case studies
Identification of genes predicted to encode relevant robust BVMOs and oxidases
Structural elucidation of relevant of BVMOs e.g. at least 3 new enzyme structures
Quorum sensing technology and micro fluidics platform available
Set of P450 enzymes with improved performance for hydroxylation of aromatics, alkenes and APIs and a set of API-sensors to enable P450 improvement
First generation production strains for robust BVMOs
First generation production strains for microbial and human P450s
Generation of a first flow cytometer based screening system for quantification of one product in the BM3 demonstration process through fluorogenic sensors
New vector and host systems for enzyme coexpression
Stakeholder analysis
H2020Robox
The European Union has selected the ROBOX proposal as H2020 project. This project has 19 partners and will be coordinated by DSM Geleen. The proposal "ROBOX: Expanding the Industrial Use of Robust Oxidative Biocatalysts for the Conversion and Production of Alcohols" took first place in the selection.
