Thermostability of P450s for Industrial Use

Good review of this essentiial feature of Cytochrome P450s for application

Highlights

• Thermostability is a desirable feature for using P450s in industrial processes
• The structures of four P450s from thermophiles have been characterized to date.
• P450s from thermophiles tend to be shorter overall than those from mesophiles.
• Extended salt-bridged and aromatic networks may stabilize the P450 fold.
• Directed evolution has been used to increase the thermostability of mesophilic P450s.