One of the problems facing potential users wishing to replicate enzyme technology is the availability of the enzyes – our partner RUG now make some ROBOX robust enzymes available:

–Eugenol oxidase (EUGO)

Robust oxidase acting on phenolic compounds

details in Nguyen et al. (2016) Chembiochem. 17:1359-1366

–Thermostable cyclohexanone monooxygenase (TmCHMO)

Robust Baeyer-Villiger monooxygenase acting on various ketones and sulfides

details in Romero et al. (2016) Angew Chem Int Ed Engl. 55:15852-15855

–Polycyclic ketone monooxygenase (PockeMO)

Robust Baeyer-Villiger monooxygenase acting on bulky ketones and sulfides

details in Fürst at al. (2017) J Am Chem Soc. 139:627-630
Groningen Enzyme & Cofactor Collection – GECCO
The biotechnology-oriented research institute GBB at the University of Groningen houses many top-notch scientists. In particular, the group of Prof. M.W. Fraaije (research group Molecular Enzymology) excels in research on discovery and engineering of redox enzymes while the group of Prof. D.B. Janssen (research group Biocatalysis and Biotransformations) is expert on a complementing selection of enzymes, such as dehalogenases, hydrolases and lyases. Publications of these two groups can be found in many renowned journals and they are highly cited and well known in the field of enzyme engineering. Extensive publications record clearly indicate large number of newly discovered and improved enzymes which altogether serve as a scientific basis for proposed initiative with a commercial purpose: