Our latest publication describes further developments at our partner RUG in fusion enzymes for co-factor recycling.
See “What to sacrifice? Fusions of cofactor regenerating enzymes with Baeyer-Villiger monooxygenases and alcohol dehydrogenases for self-sufficient redox biocatalysis”
Read more about our portfolio of cytochrome P450 enzymes in Exploring the Biocatalytic Potential of a Self‐Sufficient Cytochrome P450 from Thermothelomyces thermophila
Read our latest review article on monooxygenases
Beyond active site residues: overall structural dynamics control catalysis in flavin-containing and heme-containing monooxygenases
Read our publication “A hydroquinone-specific screening system for directed P450 evolution” This was published by our partner RWTH Aachen and describes the direct hydroxylation of benzene to hydroquinone (HQ) under mild reaction conditions that is a challenging task for chemical catalysts.
This paper describes co-immobilized biocatalyst enzymes that allows cofactor regeneration and higher yields.
See our latest paper that demonstrates the environmental metrics of our biocatalysis technology
A prospective life‐cycle assessment (LCA) of monomer synthesis: Comparison of biocatalytic and oxidative chemistry
See our VIP award winning publication on fused oxidation enzymes
“Design of Artificial Alcohol Oxidases: Alcohol Dehydrogenase–NADPH Oxidase Fusions for Continuous Oxidations”
Our latest publication from RUG/UP has applied cumulative alanine mutagenesis approach to cyclohexanone monooxygenase from Thermocrispum municipale, a flavin-dependent Baeyer-Villiger monooxygenase to demonstrate “Side-Chain Pruning Has Limited Impact on Substrate Preference in a Promiscuous Enzyme”
Our partner RUG have published a paper on how ROBOX catalysts can be used to add value to glycerol – a byproduct of biodiesel production that is treated as waste.
“Structure-based engineering of Phanerochaete chrysosporium alcohol oxidase for enhanced oxidative power towards glycerol”
See ROBOX latest publication from RUG
“Design of artificial alcohol oxidases: alcohol dehydrogenase ‐ NADPH oxidase fusions for continuous oxidations”
Our partner RUG has published a review on fusion enzyme pairing – a key technique for making some enzymes suitable for industrial application.
Enzyme fusions in biocatalysis ‐ Coupling reactions by pairing enzymes
International collaboration of ROBOX partner RUG with leading groups make breakthrough published in prestigous journal JACS – “Overriding Traditional Electronic Effects in Biocatalytic Baeyer–Villiger Reactions by Directed Evolution”
For more details see here
This result is noteworthy as these mutant enzymes result in regioselectivity that is the opposite of chemical reagents and hence offer new product opportunities.
UAB and INNO publish paper “Co‐immobilization of P450 BM3 and glucose dehydrogenase on different supports for application as a self‐sufficient oxidative biocatalyst” that represents a step forward for future cytochrome P450 BM3 industrial implementations as it facilitates scale up and enzyme recovery.
See paper here
ROBOX partners UNIMAN and UP collaborate on “The crystal structure of P450-TT heme-domain provides the first structural insights into the versatile class VII P450s” paper
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UM publish paper on synthesis of novel polymers.
ref=”http://Application of a thermostable Baeyer-Villiger monooxygenase for the synthesis of branched polyester precursors“>
UNIMAN publish paper on P450s “Cloning, expression and characterisation of P450-Hal1 (CYP116B62) from Halomonas sp. NCIMB 172: A self-sufficient P450 with high expression and diverse substrate scope”
https://www.sciencedirect.com/science/article/pii/S0141022918300383
RUG have published this joint paper in collaboration with the group of Manfred Reetz “Manipulating the stereoselectivity of the thermostable Baeyer–Villiger monooxygenase TmCHMO by directed evolution”
See paper
Our ROBOX partners at UM and RUG have been using BVMO enzymes to synthesize lactones for polymer applications
Exploring the Substrate Scope of Baeyer-Villiger Monooxygenases with Branched Lactones as Entry towards Polyesters
Full Paper
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Our partner UAB have published a ROBOX publication on immobilized self-sufficient Baeyer–Villiger monooxygenases is discussed.
An immobilized and highly stabilized self-sufficient monooxygenase as biocatalyst for oxidative biotransformations
Abstract
Our partners from Graz have published this review on the area “Human Enzymes for Organic Synthesis” which describes advancements in drug metabolites which is important for human health improvements.